Almost to tease Suns fans, Ablett was named in the side to play Port Adelaide this weekend, only to fail a fitness test just two hours later.
Earlier on Thursday, interim coach Dean Solomon said Ablett was likely to play against Port Adelaide.
“I had a really good conversation with him five minutes ago and he said he’s feeling really confident,” Solomon said.
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“You back his judgement, he’s earnt a lot of trust over the years and he knows his body better than us all.”
The dual Brownlow medallist has struggled with hamstring injuries the last few weeks and has missed their last two games.
Suns General Manager of Football Operations, Marcus Ashcroft, said it was a shame Ablett wasn’t able to prove his fitness.
“Gaz gave himself the best opportunity to play this weekend,” Ashcroft said.
“But unfortunately at training this evening he simply could not reach the speed required to be passed fit to play this weekend.”
Ablett will be replaced in the selected side by Mitch Hallahan.
Jack Bowes, who injured his hamstring during the training session has also been ruled out of the match. He will be replaced by Brad Scheer.
Alex Sexton has been added to the squad as an emergency.
Ablett’s injuries continue to add to the speculation he’ll try and move to Geelong in 2018, despite having a year left on his contract at the Suns.